Analysis of antibiotic resistance gene expression inPseudomonas aeruginosaby quantitative real-time-PCR
نویسندگان
چکیده
منابع مشابه
Real-Time PCR for Gene Expression Analysis
After discovery of polymerase chain reaction (PCR) by Dr. Kary Mullis in 1983, several different types of PCR have been invented and continually improved upon over the years. One of them called "Real-time PCR" or “fluorescence based PCR” allows us to quantitate nucleic acids obtained from cells or tissues, to compare the variable states of infection, to detect chromosomal translocations, to gen...
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The sensitivity of analysis achievable with PCR has led to the technology being adopted across a range of sectors. For many applications a quantitative result is required, which has driven the development of a range of strategies to determine the amount of starting material in a sample. Approaches such as competitive PCR and limiting dilution analysis have been used as routes to quantification,...
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Background: Acute myeloid leukemia (AML) is one of myeloid malignancies which the risk increases with age increment. It is categorized based on genetic aberrations. Some of these genetic disorders can determine minimal residual diseases (MRD) and prognosis of AML patients. Wilms tumor (WT1) over expression is found in AML patients. The aim of this study was to determine the frequency of WT1 ove...
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متن کاملQuantitative biomarker analysis of synovial gene expression by real-time PCR
Synovial biomarker analysis in rheumatoid arthritis can be used to evaluate drug effect in clinical trials of novel therapeutic agents. Previous studies of synovial gene expression for these studies have mainly relied on histological methods including immunohistochemistry and in situ hybridization. To increase the reliability of mRNA measurements on small synovial tissue samples, we developed a...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2006
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.2005.00008.x